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Search for more papers by this author. Screening for adult plant resistance in wheat to stem rust, caused by Puccinia graminis f. Although such evaluations are successful if managed properly, field ratings are time consuming, expensive, weather dependent, and open to inoculum of unwanted races or other confounding diseases.
The objective of this study was to develop a dependable system of screening the response of adult plants to stem rust under greenhouse conditions. A comparison of inoculation methods and incubation environments showed that plants inoculated with urediniospores suspended in water, followed by a 24 h dew period in a plastic chamber constructed in a greenhouse, gave the most consistent results.
Measurements of response type, stem rust severity, and frequency in follow-up experiments indicated that the most reliable infection was obtained when plants sprayed with 1. Depending on the race, composite interval mapping showed flag leaf infection type to be significantly influenced by regions on chromosomes 6A, 6D, and 7D.
This study showed that phenotyping and genetic analysis of especially major effect stem rust resistance in adult wheat plants is possible and reproducible under controlled conditions in a greenhouse. Stem rust of wheat Triticum aestivum L. This discovery, in particular the occurrence of virulence for the commonly used Sr31 resistance gene, has since initiated a global effort to combat the disease McIntosh and Pretorius Although fungicides can be used to control wheat stem rust Wanyera et al.
Thus, the deployment of resistant cultivars is widely accepted as the best way to reduce inoculum levels and to prevent or control the disease, especially for resource-poor farmers. Since rust pathogens have the ability to adapt and acquire virulence for resistance genes, the ongoing identification, characterization, and utilization of sources of resistance are needed.